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How to Analyze UK NEQAS Samples:

UKNEQAS samples are stabilized whole blood samples with light scatter and fluorescence staining properties that are different from fresh blood specimens and as such may not satisfy the automatic gating algorithm defined by the instrument software.  The instrument software may flag the analysis with error identifying questionable sample integrity. In this instance, a manual gating should be applied to the analysis. The gating of the populations should be reviewed to ensure that the population clusters are gated to include the entire cluster without any interfering background and debris events.

Example of software-defined automatic gating of specimen of
questionable sample integrity.

Figure 1

 QC Messages:

Code 3: Sample integrity questionable. Visually inspect.

Example of manual gating analysis.

Figure 2

QC Messages: 

Code 7: Manual Gate is in effect